Uman National University | today: 05/23/2026
| Author(s) |
Lyubchenko A.I., Candidate of Agricultural Science, , Uman National University of Horticulture, Ukraine Lybchenko I.O., postgraduate, , Uman National University of Horticulture, Ukraine Ryabovol Y.S., , postgraduate, Uman National University of Horticulture, Ukraine Сержук О. П., , , |
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| Category | The Agronomy | ||
| year | 2026 | issue | Issue 108 part 1 |
| pages | 367-376 | index UDK | 581.522.4-047.44:581.143.6:546.33’131]:633.85 | DOI | 10.32782/2415-8240-2026-108-1-367-376 (Link) |
| Abstract | One of the most harmful environmental stress factors is soil salinity, caused by the presence of high concentrations of salt solutions in the soil absorption complex. Salinization leads to deterioration of the agrophysical and agrochemical properties of the soil and reduces plant viability and productivity. Crop production on saline soils requires the implementation of a complex of reclamation and management measures. The introduction of salt-tolerant varieties is a key prerequisite for obtaining stable yields of agricultural crops in such regions. Incorporating biotechnological approaches into the breeding process for developing salt-tolerant varieties significantly increases the efficiency of creating genotypes with desirable traits. The aim of the study was to determine the stress effect of sodium chloride on the callus culture of camelina sativa. To create an in vitro selective system, sodium chloride (NaCl) was added to the Murashige and Skoog nutrient medium at concentrations of 0.25, 0.5, 0.75, 1.0, 1.25, and 1.5 %. Morphogenic callus tissue of the camelina varieties Yevro 12, Klondike, Peremoha, and Stepovyi 1 was used in the study. In each experimental variant, 50 microcalli were cultured. The experiment was conducted in four replicates. The biomaterial was cultivated at a temperature of 24–25 °C, relative air humidity of 75 %, and a 16-hour photoperiod. At the end of each passage, the viability of the microcalli, growth intensity, and morphogenic characteristics of the induced biomaterials were evaluated. Based on the obtained data, the stress susceptibility index (SSI) was determined, characterizing the viability of the genotype under stress conditions relative to the average response of all studied samples. The conducted studies revealed the stress effect of sodium chloride on the callus tissue of camelina sativa. Chloride salinity affected the viability of microcalli, proliferation activity, and maintenance of morphogenic activity. An NaCl concentration of 1.0–1.25 % was found to be optimal for ranking genotypes according to salt tolerance and for conducting cell selection for salt tolerance. The highest resistance to chloride salinity was observed in microcalli obtained from the varieties Peremoha and Stepovyi 1. | ||
| Key words | Camelina sativa, variety, salinity stress, sodium chloride, callus, in vitro culture | ||