Uman National University | today: 05/23/2026

Influence of nutrient medium on rooting of plants of soft winter wheat using aerohydroponics

Author(s) Ryabovol Y.S., , postgraduate, Uman National University of Horticulture, Ukraine
Фесько М. В., , ,
Category The Agronomy
year 2026 issue Issue 108 part 1
pages 221-227 index UDK 631.527.581.143:633.11
DOI 10.32782/2415-8240-2026-108-1-221-227 (Link)
Abstract Ensuring optimal conditions for the growth and development of plants, similar to the conditions for realizing their genetically programmed productivity potential, should be implemented through control and regulation systems. Aerohydroponic systems are the most effective in this context, as they can provide a wide range of regulation of environmental factors, making it possible to design compact and economically functional units for growing biomaterial. The use of such systems creates a stable microclimate that contributes to the realization of the genetic potential of plants, while at the same time shortening the period of obtaining new starting material. The creation of optimized cultivation conditions, with the possibility of programmed control of the main abiotic factors of the environment, ensures the production of new breeding material with intensive development of vegetative mass and roots. When using aerohydroponic technology, the composition of the nutrient solution is of crucial importance, which differs depending on the type of plant, the period of ontogenetic development and the environmental conditions of the agrocenosis, where the biomaterial will be transferred. The purpose of the research was to develop a nutrient medium for aerohydroponic systems with intensification of rhizogenesis induction and adaptation of plant material of soft winter wheat when transferring plants from an isolated in vitro culture to natural environmental conditions. The research was conducted during 2023–2026 in the educational and scientific laboratory of biotechnology of the Uman National University.The starting material was propagated in vitro hybrid samples of soft winter wheat, obtained by culturing isolated immature and mature embryos, which ensures maintenance of genetic homogeneity and reduction of the level of somaclonal variability. In the process of research, the composition of the nutrient medium for the rooting of soft winter wheat plants using aerohydroponics was selected. It has been confirmed that growth regulators of auxin nature, in particular naphthylacetic acid and heteroauxin, significantly affect rooting and the intensity of root system development of soft winter wheat using an aerohydroponic system. The introduction into the substrate of half the concentration of macro- and microelements according to the Murasige-Skug prescription, 1.0 mg/l of naphthylacetic acid, 0.5 mg/l of heteroauxin, 0.3 mg/l of 6-benzylaminopurine and 1.0 mg/l of gibberellic acid provides stimulation of rhizogenesis and optimal development of the plant root system. The rate of rooting and the intensity of plant development in the conditions of aerohydroponics significantly depends on the genotype of the source material
Key words soft winter wheat, initial material, in vitro culture, genotype, aerohydroponics, nutrient medium, growth regulators, rhizogenesis, root system
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