Uman National University | today: 12/25/2025
| Author(s) |
Фесько М. В., , , Бабій М. М., , , |
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|---|---|---|---|
| Category | The Agronomy | ||
| year | 2025 | issue | Issue 107 part 1 |
| pages | 286-293 | index UDK | 631.527.581.143:633.11 | DOI | 10.32782/2415-8240-2025-107-1-286-293 (Link) |
| Abstract | The aerohydroponic system, as a high-tech model of substrate-free cultivation on standardized nutrient media, is an effective tool for researching the physiological and biochemical reactions of intact plants aimed at identifying the influence of endogenous growth regulators and exogenous environmental factors on morphogenesis with a high level of control of cultivation parameters. The aim of the research was the development of a biotechnological method of intensification of rhizogenesis and increase of the adaptation potential of cloned lines of soft winter wheat using aerohydroponic technology and modeling of physiological and genetic mechanisms of root system formation in the conditions of transfer of plants from in vitro to ex vitro culture. The source material was hybrid plants of soft winter wheat, microcloned in vitro, obtained using germ culture, which ensures preservation of genotypic stability and minimization of somaclonal variability. The high efficiency of using aerohydroponic technologies for rooting and adaptation of cloned plants of soft winter wheat has been confirmed іn the process of research. A modified composition of the nutrient solution was developed, with the addition of ½ MS (macro- and microelements), 1.0 mg/l indoleacetic acid, 0.5 mg/l heteroauxin, 0.3 mg/l 6-benzylaminopurine and 1.0 mg/l gibberellic acid.The main stages of the morphogenesis of the root system using aerohydroponic cultivation are highlighted, which include the phases of adaptation, induction of rhizogenesis, formation of lateral roots and development of the twining root system. The optimal parameters for obtaining acclimatized plants with a branched, physiologically active root system have been determined, which ensures a reduction in the time of obtaining and an increase in the quality of breeding material, suitable for further use in genetic selection programs for the creation of initial forms and varieties of soft winter wheat. | ||
| Key words | soft winter wheat, genotype, the source material, aerohydroponic technologies, culture in vitro, nutrient medium, rhizogenesis | ||