Uman NUH | today: 12/21/2024
| Author(s) |
Войтовська В.І., , , Lubych V.V., Candidate of Agricultural Science, Lecturer of Department of Technology of Storage and Processing of Grain, Uman National University of Horticulture, Ukraine |
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| Category | The Agronomy | ||
| year | 2024 | issue | Issue 105 part 1 |
| pages | 192-201 | index UDK | 602.4:606:632.4:633.6 | DOI | 10.32782/2415-8240-2024-105-1-192-201 (Link) |
| Abstract | Goal. To determine the optimal concentrations and to obtain a tolerant source material of stevia to alternariosis in in vitro culture. Methods. Laboratory, biotechnological, measuring, calculation and comparison, analysis, statistical. The results. It was established that, regardless of the concentration of culture fluid filtrate, the highest rates of viable shoots were noted in the Bereginya variety (from 12 to 89 %), and the lowest in the Halyna variety (from 8 to 66 %) and line № 16 (from 5 to 78 %). A high percentage of viable stevia shoots was obtained at a concentration of 30 % – from 68 to 90 %. As in previous studies the highest indicators were in the Bereginya (st.) and Slavutych varieties – 90 %. Studies indicate that increasing the filtrate content to 35 % was not critical and allowed to obtain a high percentage of viable stevia shoots – from 66 to 89 %. The following indicators were obtained from the studied materials: varieties Bereginya (st.) and Slavutych – 89 and 87 %, lines – № 3 – 85 %, № 11–83 %, № 14 – 80 %, № 16 – 78 %. Cultivation for 14 days with 15 and 20 % culture fluid filtrate ensured the formation of the number of shoots in stevia varieties Bereginya (st) and Slavutych – 11–13 pcs. and 12 pcs., Galina – 8 and 7 pcs., lines № 3 – 10 pcs., № 11 – 12 and 10 pcs., № 14–11 and 8 pcs., № 16 – 10 pcs. It is appropriate to note the Halyna variety, in which the number of shoots was 4–5 pcs. and line № 16, in which this indicator was in the range of 5–8 pcs., and the highest indicators were in the Bereginya variety (st) – 10 pcs. All stevia shoots that were tolerant to the filtrate of the culture liquid of the fungus were selected, propagated and rooted and tested in the field. Conclusions. It was established that at a concentration of culture fluid filtrate 90–130 %, it significantly affects all parameters of the investigated materials of stevia and causes its death. It was determined that the harmful concentration of culture fluid filtrate is 40 and 50 %, with the first from 31 to 63 % of shoots, and with the second from 12 to 36 %, in varieties and lines from 24 to 31 % of shoots die. It has been studied that the number of necrotic shoots begins to increase during the long-term cultivation of shoots on the 7th day. It was established that increasing the cultivation to 14 days with concentrations of 35–45 % ensures obtaining shoots with a length 5–12 cm, and with a decrease in the amount of filtrate, the length of the shoots increases to 19 cm. | ||
| Key words | varieties, lines, concentration of the filtrate of the culture liquid of the mushroom, shoot formation, length | ||