Uman National University | today: 06/27/2025
| Author(s) |
Карпенко В. В., , , Заболотна А. В., , , |
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|---|---|---|---|
| Category | The Agronomy | ||
| year | 2025 | issue | Issue 106 Part 1 |
| pages | 602-608 | index UDK | 612.015.1:[633.11+633.14]:632.93 | DOI | 10.32782/2415-8240-2025-106-1-602-608 (Link) |
| Abstract | Introduction. The key mechanism of action of biological preparations on plants is their effect on enzyme activity, which determines the direction of metabolic processes, resistance to stress and the ability to absorb nutrients. One of the reactions of plants to stress is the formation of reactive oxygen species, which the plant organism inactivates with the participation of enzymes. Research methods. The activity of enzymes of the oxidoreductase class (catalase (CAT, EC 1.11.1.6), peroxidase (PX, EC 1.11.1.7), polyphenol oxidase (PPO, EC 1.10.3.1)) was determined in samples of winter triticale leaves, selected in the field, in the corresponding phases of plant development, according to generally accepted methods. Results. During the experiment, changes in the activity of enzymes of the oxidoreductase class of winter triticale plants were detected depending on the type and method of use of the preparations. Thus, on average over the years of research in the phase of exit into the tube under the seed bacterization with Melanorhiz, the activity of catalase increased by 11%, peroxidase by 5%, and polyphenoloxidase by 7% compared to the control, under the pre-sowing treatment of seeds with Biozlak, the activity of the studied enzymes exceeded the indicators of the control variant by 9, 4, and 5%, respectively. А spraying of vegetative plants with Bactofit at the norms of 2,0; 2,5, and 3,0 l/ha contributed to an increase in the activity of catalase compared to the control by 10, 23, and 32%, peroxidase by 5, 8, and 15%, and polyphenoloxidase by 4, 11, and 17%, according to the norms of the biofungicide. The use of the specified norms of Bactofit against on the background of pre-sowing seed bacterization with Biozlak contributed to the increase in catalase activity compared to the control variant. Among all the variants of the experiment, the highest enzymatic activity was provided by the use of Bactofit (2,0; 2,5 and 3,0 l/ha) on the background of pre-sowing seed bacterization with Melanoriz. In these variants of the experiment, catalase activity increased against the control variant by 23, 36 and 46%, peroxidase – by 14, 18 and 22%, polyphenoloxidase – by 26, 42 and 49%. In the flowering phase of winter triticale, the maximum enzyme activity was detected compared to the tube exit phase. However, as in the previous phase, the highest enzyme activity was also observed after spraying crops with Biozlak on the background of pre-sowing seed bacterization with Melanoriz. Conclusions. Thus, the highest activity of enzymes of the oxidoreductase class was established with the complex use of preparations – the bacterization of seeds with Melanorhiz (1,0 l/t) followed by the use of the biofungicide Baktofit (3,0 l/ha) on this background, which is obviously due to the significant activation of plant-microbial interactions, due to which plant nutrition and the course of metabolic processes in them improve, the basis of which is enzymatic activity. | ||
| Key words | enzyme activity, catalase, peroxidase, polyphenol oxidase, biological preparations, winter triticale | ||