Uman National University | today: 12/25/2025
| Author(s) | Березовський А. А., , , | ||
|---|---|---|---|
| Category | The Agronomy | ||
| year | 2025 | issue | Issue 107 part 1 |
| pages | 421-428 | index UDK | 631.461:633.16"321":631.86/.87 | DOI | 10.32782/2415-8240-2025-107-1-421-428 (Link) |
| Abstract | The results of studies on the separate and combined effects of the biological preparations Biozlak (seed treatment 1.0–1.5 l/t) and Baktiva (250 g/ha) on the functioning of individual groups of the rhizosphere microbiota of spring naked barley, in particular nitrifying, cellulolytic microorganisms, Azotobacter, and Clostridium pasteurianum. The research was conducted in field and laboratory conditions at the Department of Biology of Uman National University during 2024–2025. The effect of the biological preparations Biozlak and Baktiva was studied in crops of the spring naked barley variety Natir, which is used for food and feed. Field experiments were set up using a systematic method with three repetitions. Naked barley seeds were treated with the biological preparation Biozlak one day before sowing. Against the background of treating naked barley seeds with Biozlak, the crops were sprayed with the biological preparation Baktiva at a rate of 250 g/ha using a battery-powered backpack sprayer, based on a working solution consumption rate of 200 l/ha. The abundance of individual groups of microbiota in the rhizosphere of spring naked barley was recorded during the stem elongation and heading stages of the crop. Soil samples were taken in accordance with generally accepted methods. The abundance of rhizosphere microorganisms – nitrifying bacteria, Clostridium pasteurianum – was determined by sowing soil suspensions of appropriate dilutions on S. M. Vinogradsky's medium, and cellulolytic microorganisms – on O. O. Imschenetsky and L. I. Solntseva, Azotobacter – on Ashby's nitrogen-free nutrient medium, counting the soil lumps overgrown with colonies. The number of microorganisms was expressed in thousands of cells per 1 g of absolutely dry soil. The use of biological preparations Biozlak and Baktiva separately and in combination contributed to a more active (compared to the control) development of certain groups of microorganisms in the rhizosphere of naked barley. The largest number of individual groups of microorganisms in the rhizosphere of naked barley crops was observed in the experiment variant using Biozlak (1.5 l/t) for seed treatment before sowing, followed by spraying the crops with Baktiva (250 g/ha), where the average excess over the control for the phases of crop development was 31–35% for nitrifying bacteria, 33–44% for cellulolytic bacteria, 25–26% for bacteria of the genus Azotobacter, and 30–40% for bacteria of the genus Clostridium pasteurianum. | ||
| Key words | spring naked barley, biological preparations, microorganisms | ||