The article presents the research results on the effects of the biopreparation Bioneostim (1.0 l/t), used with various methods of applying the plant growth regulator Vermistim D (seed treatment before sowing – 7.0 l/t, crop spraying – 8.0 l/ha), on the number and mass of nodules on the root system of sowing grasspea (Lathyrus sativus). The study aimed to determine the impact on the symbiotic activity of sowing grasspea from pre-sowing seed inoculation with a combination of biopreparation and plant growth regulator, followed by post-emergence application of the plant growth regulator.
The research was conducted under field conditions by the Department of Biology at Uman National University of Horticulture from 2022 to 2024. The effects of the biopreparation Bioneostim and the plant growth regulator Vermistim D were studied in crops of the grasspea variety “Ivolga”. Field experiments were conducted using the systematic method with three replicates. The experimental design included variants with seed treatment before sowing with Bioneostim at a rate of 1.0 l/t, both separately and in combination with the plant growth regulator Vermistim D (7.0 l/t – seed treatment and 8.0 l/ha – foliar treatment). Grasspea seeds were treated with biopreparation, the plant growth regulator, and their mixtures one day before sowing. On the background of seed treatment with Bioneostim and Vermistim D, crops were sprayed with Vermistim D at a rate of 8.0 l/ha during the tillering phase, using 200 l/ha of the working solution.
The experimental data indicate that the formation of the symbiotic apparatus in sowing grasspea depended on both weather conditions and the combination of the studied preparations. The most active formation of the symbiotic apparatus occurred in the experimental variant with pre-sowing treatment of seeds with a mixture of Bioneostim and Vermistim D, followed by post-emergence application of Vermistim D. On average, over the years of research, this led to an increase in the number of nodules depending on the development phase of the crop by 19–24 per plant and 28.2–227.9 mg per plant. The increase in the number and mass of nodules on the root system of sowing grasspea was related to the direction of physiological and biochemical processes in the plants, which, in turn, were influenced by the combination of biopreparation and plant growth regulators in the cultivation technology.