Uman NUH | today: 04/12/2024
| Author(s) |
Ryabovol L.O., Doctor of Agricultural Sciences, Professor, Professor of Department of Genetics, Plant Breeding and Biotechnology, Uman National University of Horticulture, Ukraine Ryabovol Y.S., , postgraduate, Uman National University of Horticulture, Ukraine Ненька О.В., , , |
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| Category | The Agronomy | ||
| year | 2023 | issue | Issue number 102. Part 1 |
| pages | 165-171 | index UDK | 631.52:581.143.5:633:78 | DOI | 10.32782/2415-8240-2023-102-1-165-171 (Link) |
| Abstract | Preservation of genotypes of cross-pollinated crops, in particular sugar beet, is an important issue of the breeding process. The use of biotechnological methods ensures the preservation and reproduction of the necessary amount of the created material and its use in selection for the purpose of obtaining new varieties and hybrids of agricultural crops. In the article it is analyzes the influence of the genotype on the process of microclonal reproduction of various breeding forms of sugar beet. It was confirmed that the induction of the development of the primary explant, the intensity of development and growth of biomaterial, the rooting of plants is a genetically determined factor. It was proven that fertility restorers in isolated culture are characterized by a higher regeneration capacity compared to emergency lines. The best indicators were recorded for the cultivation of explants of the sample MM 2 × 105, which induced intensive establishment of adventitious meristematic buds at the level of 90,2 %. Data analysis also indicates a significant difference in the intensity of shoot formation between different selection samples. The largest amount of material was induced during the cultivation of fertility restorers (10.8–12.7 pcs. of shoots per one explant), the smallest – from ChS-lines (4.3–5.8 pcs.). It is necessary to point out the high intensity of growth and development of clones obtained from hybrid material. The growth of biomaterial on the 30th day of cultivation reached 41.6 mm, which made it possible to obtain plants with maximally developed vegetative photosynthetic organs (5–7 leaves per clone) in a short period of time. The share of rooted material was reliably high and reached 95,0 % on average for individual genotypes. The performed cytological analysis confirmed the genetic identity of the obtained clones with respect to the original material by 94,2 %. | ||
| Key words | sugar beet, genotype, microclonal propagation, regeneration, adventitious buds | ||