Uman NUH | today: 12/21/2024
| Author(s) | Ковальова І. А., , , | ||
|---|---|---|---|
| Category | The Agronomy | ||
| year | 2020 | issue | Issue number 97. Part 1 |
| pages | 145-154 | index UDK | 634:836.1.631.538 | DOI | 10.31395/2415-8240-2020-97-1-145-154 (Link) |
| Abstract | Based on the grapevine genotypes of NSC “Tairov Research Institute of Viticulture and Wine-Making” analysis, traits collections have been created and registered, representing genotypes for breeding programs to obtain new varieties resistant to abiotic and biotic environmental factors (frost and phytopathogens) under conditions implementation of climate change scenarios, varieties with improved quality traits and seedless varieties. Genetic ordering of traits collections has begun on the basis of varieties molecular identification using microsatellite DNA markers (from 5 to 9 MC loci) and identification using DNA markers of genes of interest or gene complexes (primarily a trait of seedlessness). Microsatellite analysis made it possible to identify a total of about 80 genotypes, mainly the varieties breeded at the NSC “Tairov Research Institute of Viticulture and Wine-Making”, belonging to the traits collections of resistance and quality. Visual sanitary control of three traits collections showed a practical absence of virus diseases and grapevine crown gall disease symptoms. Sometimes symptoms similar to the phytoplasma infection manifestation were found. The data of virus diseases agents identification by ELISA showed the absence the most harmful viruses — grapevine fanleaf virus (GFLV) and grapevine leafroll virus 1 (GLRaV I) on 19 samples tested. From samples of resistance traits collection one sample was positive on grapevine leafroll virus 3 and two were positive on grapevine fleck virus (GfkV). These samples should be eliminated from further propagation and genetic material moving between germplasm repositories. The causative agent of grapevine crown gall disease (Agrobacterium vitis) was not detected on 14 samples of 3 traits collections tested by PCR. It has been determined that the sanitary status of grapevine traits collections practically corresponds to the European minimum sanitary requirements, genetic ordering of characteristic collections will be continued. | ||
| Key words | Based on the grapevine genotypes of NSC “Tairov Research Institute of Viticulture and Wine-Making” analysis, traits collections have been created and registered, representing genotypes for breeding programs to obtain new varieties resistant to abiotic and biotic environmental factors (frost and phytopathogens) under conditions implementation of climate change scenarios, varieties with improved quality traits and seedless varieties. Genetic ordering of traits collections has begun on the basis of varieties molecular identification using microsatellite DNA markers (from 5 to 9 MC loci) and identification using DNA markers of genes of interest or gene complexes (primarily a trait of seedlessness). Microsatellite analysis made it possible to identify a total of about 80 genotypes, mainly the varieties breeded at the NSC “Tairov Research Institute of Viticulture and Wine-Making”, belonging to the traits collections of resistance and quality. Visual sanitary control of three traits collections showed a practical absence of virus diseases and grapevine crown gall disease symptoms. Sometimes symptoms similar to the phytoplasma infection manifestation were found. The data of virus diseases agents identification by ELISA showed the absence the most harmful viruses — grapevine fanleaf virus (GFLV) and grapevine leafroll virus 1 (GLRaV I) on 19 samples tested. From samples of resistance traits collection one sample was positive on grapevine leafroll virus 3 and two were positive on grapevine fleck virus (GfkV). These samples should be eliminated from further propagation and genetic material moving between germplasm repositories. The causative agent of grapevine crown gall disease (Agrobacterium vitis) was not detected on 14 samples of 3 traits collections tested by PCR. It has been determined that the sanitary status of grapevine traits collections practically corresponds to the European minimum sanitary requirements, genetic ordering of characteristic collections will be continued. | ||