Uman NUH | today: 04/14/2024

Selection of the optimal sterilization schedule for explants of erectoid forms of corn

Author(s) Ryabovol L.O., Doctor of Agricultural Sciences, Professor, Professor of Department of Genetics, Plant Breeding and Biotechnology, Uman National University of Horticulture, Ukraine
Ryabovol Y.S., , postgraduate, Uman National University of Horticulture, Ukraine
Білокур Ю. В., , ,
Category The Agronomy
year 2020 issue Issue number 97. Part 1
pages 45-51 index UDK 57.085.2: 633.15
DOI 10.31395/2415-8240-2020-97-1-45-51 (Link)
Abstract The article presents the results of studies to optimize the sterilization technique for seedlings of the initial forms of heterotic hybrids of maize when administered in vitro. On the surface of the plant there is a significant number of various microorganisms that can multiply on a nutrient medium. Therefore, sterilization of plant material is one of the most important stages of in vitro propagation technology. The aim of the research was to develop a procedure for sterilizing the apical meristem of maize seedlings when the material is introduced into isolation culture. To solve the problem, the task was to clarify the features of the use of common and new sterilizers and the selection of optimal modes for effective sterilization of seedlings. Seedlings of a number of maize lines and hybrids were used as explants. The purified and washed plant material was placed in cups with 70 % ethyl alcohol for 1–2 min, and then transferred to the basic solution for sterilization. After sterilization, the material was washed 5–8 times in sterile distilled water. Studies have established the features of the use of a number of sterilizers and selected the optimal modes for effective sterilization of seedlings of forms of corn with erectoid placement of leaves. It was found that sodium hypochlorite is characterized by a high toxic effect on living organisms, in high concentrations causes a significant amount of explant necrosis. The duration of treatment of corn seedlings with hydrogen peroxide of 3 % concentration did not affect the sterilization efficiency. The most effective sterilizing substance for the introduction of seedlings in an isolated culture was determined 0.1 % solution of potassium permanganate exposure of 10 minutes, which allows to obtain up to 86 % of viable seedlings. With an increase in the sterilization exposure to 15 and 20 minutes, the yield of sterile explants significantly decreased and amounted to 65 and 48 %, respectively. It was noted that when using potassium permanganate, the amount of infected material was the lowest in the experiment and varied within 0.6–1.8 %.
Key words corn, in vitro, explant, sterilization regulations, sterilizers, concentration, exposure
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