Uman NUH | today: 05/24/2019

Influence of the modified growing medium on microcloning of in vitro camelina plants

Author(s) Lybchenko I.O., postgraduate, , Uman National University of Horticulture, Ukraine
Ryabovol L.O., Doctor of Agricultural Sciences, Professor, Professor of Department of Genetics, Plant Breeding and Biotechnology, Uman National University of Horticulture, Ukraine
Lyubchenko A.I., Candidate of Agricultural Science, , Uman National University of Horticulture, Ukraine
Category The Agronomy
year 2018 issue Випуск №92. Частина 1
pages 133-141 index UDK 581.151+581.165.7:633.85
DOI
Abstract Camelina sativa is a promising agricultural crop which has food, technical and energy significance. A short period of vegetation, resistance to diseases and pests and unpretentiousness to the cultivation conditions determine the high economic efficiency of its production. The limiting factor of expanding areas for this crop is lack of high-yielding varieties. Recently, biotechnological methods, in particular microclonal propagation, are used in selection and genetic studies and seed production. The effectiveness depends on a number of factors, the main of which are the composition of the nutrient medium, concentration and ratio of growth regulators in it. This question is poorly known for camelina. In our studies, basic nutrient media were modified by growth regulators of auxin (2.4-dichlorophenoxyacetic acid) and cytokinin (6-benzylaminopurine) nature in various concentrations and ratios. Explants were apical meristems of camelina sprouts of Stepnoi 1 variety. In the course of conducted studies, it is found that a horizontal type of propagation is characteristic for camelina. The biomaterial propagation is due to the development of lateral adventive shoots. For intensive propagation of in vitro plant material of camelina the presence of auxins and cytokinins in the growing medium is a prerequisite. The highest propagation rate (9.3 micro shoots) was observed on the Murasige-Skoog growing medium with the modification of ІАА and 6-BAP at concentrations of 1.0 mg/l. The increase in cytokinin content to 1.5 mg/l slightly changes indicators of the morphogenetic activity. On the Gamborg and Schenk-Hildebrant growing media at the indicated ratio of growth regulators in vitro propagation rate was 26–31% lower. The content and ratio of growth regulators in the growing medium influence mostly the intensity of micro propagation of сamelina sativa in vitro.
Key words сamelina sativa, plant growthregulators, nutrient medium, microclonal reproduction, in vitro
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